Acquisition¶

Use this tab to configure complete multi-dimensional acquisition sequences across all available dimension modules.

To set up a multi-dimensional acquisition:

1. Select the required dimension(s) from the panel.
2. Choose the appropriate data processor.
3. Specify the save directory for the output files.
4. Edit a description of your experiment.
   • a. Click Generate to add acquisition sequence description and/or Write your own experiment description.
   • b. Confirm to close the window.
5. Launch the acquisition.

All dimensions share a consistent interface. The UI controls for configuring dimensions include:

Icon	Description
	Inactive: The sequence excludes the dimension. Click to activate.
	Active: The sequence includes the dimension. Click to deactivate.
	Configured: The dimension's parameters are set. Here, 11 images will be acquired for this dimension.
	Edit: Click to configure the dimension settings.
	Reorder: Drag and drop to change the dimension execution order. The dimension order defines the nesting loop of the sequence, executing from top to bottom.

Dimension Execution Order¶

The order of the active dimensions dictates the nested loop structure of the acquisition sequence. Use the Reorder handle to drag and drop dimensions to change their execution order.

Dimensions at the top of the list act as the outer loops of the sequence, progressing more slowly. Dimensions positioned lower in the list act as the inner loops, executing more frequently. For example, if Time is placed above Channels, the system acquires all channels for a single time point before progressing to the next time point. Conversely, if Channels is placed above Time, the system acquires the entire time-lapse sequence for the first channel before switching to the next channel. Permuting these dimensions correctly is critical, as it directly determines how the hardware coordinates the image acquisition.