Checks whether the optional Method attribute is present.
This field captures the approach used by this TIRF Hardware Module to produce TIRF illumination. In Objective-based TIRF, the excitation lightpath and the emission channel share the same optics, which provides high signals but might result in high background. In Prism-based TIRF excitation light enters the dovetail prism, reflects at the glass/water interface and escapes through the opposite facet without interferring with the emission channel . In Lightguide-based TIRF, excitation light enters the lightguide, reflects multiple times from its top and bottom surfaces to generate wide-area evanescent waves and escapes from the opposite side of the lightguide without interferring with the emission channel.
Returns:
| Type |
Description |
boolean
|
true if the optional attribute has been set, false otherwise
|