Configuration

- Activate or deactivate Tirf using the switcher.
- Select Tirf Azimuthal (circle illumination) if you need.
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- If Tirf azimuthal is activated, you will see the diagram of the circle corresponding to the laser scanning in the Tirf angle.
- If Tirf Azimuthal is not activated, you will see the laser position (orange dot) and you can adjust the coordinates by changing the Tirf Angle value.
- Adjust Tirf radius if you need (corresponds to the angle of the laser at lens outlet for WF - HILO - TIRF).
- FRAP can be activated by clicking on the switcher.
Important: Those parameters (Tirf Azimuthal or point, Angle and Radius)
need to be saved in the Tirf channel for Tirf Imaging