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Configuration

tirf_configuration

  1. Activate or deactivate Tirf using the switcher.
  2. Select Tirf Azimuthal (circle illumination) if you need.

    1. If Tirf azimuthal is activated, you will see the diagram of the circle corresponding to the laser scanning in the Tirf angle.
    2. If Tirf Azimuthal is not activated, you will see the laser position (orange dot) and you can adjust the coordinates by changing the Tirf Angle value.
  4. Adjust Tirf radius if you need (corresponds to the angle of the laser at lens outlet for WF - HILO - TIRF).
  5. FRAP can be activated by clicking on the switcher.
Important: Those parameters (Tirf Azimuthal or point, Angle and Radius) need to be saved in the Tirf channel for Tirf Imaging