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Positions

This dimension is dedicated to image different XYZ positions from a sample. You can switch from this dimension to the Tiling dimension by the button Switch to Tiling.

positions_dimension

To use the Positions dimension:

  1. Move the stage and the focus to the desired position.
  2. Click on Add position to add the XYZ coordinates in the position list.
  3. Make other adjustments or manipulations if necessary:
    1. Add position: add a new position/s in the position list;
    2. Remove all: delete all position from the position list;
    3. Move Strategy: When performing a multiposition acquisition with a significant distance between two positions, two options are available for the stage moving between these two positions:
      • Fastest: Move directly from position A to position B;
      • XY Split: Move between 2 positions with small steps. Indicate the step by filing the Step (µm);
    4. Tag: Users can add names to the selected positions;
    5. X-Axis/Y-Axis: change/adjust position if necessary;
    6. Focus: Use the focus value used for the selection of the positions. If the checkbox is not selected, the current focus value of the microscope will be used.
    7. Move to: move the stage to this position;
    8. Get: update coordinate of the position by the actual XYZ position of the system;
    9. : delete this position from the position list.
  4. When all positions are set up, click on Confirm.