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Imaging
From core solution to custom techniques
Inscoper I.S.
Incorporating a specially-designed device, Inscoper I.S. provides a new user experience with improved technical performance, system integration and ease-of-use.
Acquisition
This tab allows you to set the entire acquisition sequence, using all the system's dimension modules.
Dimensions
Z-stack
The volumetric imaging with Inscoper I.S. can be performed using the Z-Stack dimension.

Imaging
From core solution to custom techniques
- Inscoper I.S.
  Incorporating a specially-designed device, Inscoper I.S. provides a new user experience with improved technical performance, system integration and ease-of-use.
  - User journey
  - Getting started
    When you launch the Inscoper I.S. software, the start window will appear. You will have access to the software version number and can choose from several options to begin your user journey:
  - Display settings
    To access the interface settings, click on Settings > Display Settings.
  - Configuration
  - Acquisition
    This tab allows you to set the entire acquisition sequence, using all the system's dimension modules.
    - Dimensions
      - Time
        The Time dimension is used to run time-lapse and image cells over a long period of time.
      - Multichannels
        This dimension is useful to select two (or more) channels and add them in the acquisition sequences.
      - Positions
        This dimension is dedicated to image different XYZ positions from a sample. You can switch from this dimension to the Tiling dimension by the button Switch to Tiling.
      - Tiling
      - Multiwell plate mode
        The Inscoper software has a strong feature for imaging multiwell plates and for some applications including High Content Imaging.The Multiwell plate mode of the Inscoper I.S. is compatible with all commercial or homemade supports (multi-well, multi-slide, etc.). An option Well Plate Editor allows you to simply add a large panel of supports. Click here to set it up.
      - Z-stack
        The volumetric imaging with Inscoper I.S. can be performed using the Z-Stack dimension.
      - Software autofocus
        The Inscoper I.S. has a software autofocus mode to keep samples at the focus throughout the acquisition sequence.
      - Multi-sequence acquisition
        When an acquisition sequence is ready, it is possible to launch it or to add others to create a multi-sequence acquisition.
      - Data Processor
      - Saving images and metadata
    - Data export
    - Semi-automated feedback microscopy feature
      The Inscoper I.S. can be used to perform some feedback microscopy experiments. For example, you can image a large sample using the tiling dimension. Then you can select a few areas of interest on this large image and automatically import them into a new sequence.
  - Visualization
- scanFRAP
- TIRF
- fastFLIM
- liveRATIO
- MAICO I.S.

Z-stack

The volumetric imaging with Inscoper I.S. can be performed using the Z-Stack dimension.

If necessary adjust the focus and the step.
Select the mode you want to use:
- Set the Min/Max Plane (limits of your stack) by clicking on the Get button.
- Set the Center Plane (middle of your stack) by clicking on the Get button, setting up the Volume of your ZStack and, if necessary, checking the option Center First.
Indicate the Stack Step (between 2 focal plans) and the Stack Size (number of plans).

Notice: If you set your ZStack with the center plan, the Volume, Step and Size are linked.

Fastpath: For the step size you can help you with the Nyquist Step Calculator: Indicate the emission wavelength, the numerical aperture of your objective and the refractive index of your objective immersion medium. Once all information is set up, click on Confirm to validate your settings. The step size will be automatically calculated.
Specify the desired scanning direction: Bottom to Top or Top to Bottom.
Once you have finished editing the dimension, click on the Confirm.