Acquisition

  1. Wavelengths set up
    1. In the Acquisition tab, go to Multichannels dimension.
      liveratio_multichannels
    2. Select two or more wavelengths.
  2. Data processing
    1. Select the Ratiometric imaging_Despeckle in the Data processing and Chart drop-down list.
    2. Click on the Edit Parameters to set and to optimize the ratiometric module.
      liveratio_data_processing
    3. Select the ratio to measure during the experiment.
    4. Check if you want to use the module to remove the background noise.
    5. If checked, select a ROI without any signal.
    6. If checked, select the number of times the standard deviation will be considered for the calculation.
    7. Select the filter type to apply (median or average).
    8. Select the radius to consider for this filter.
    9. Click on Confirm.
      liveratio_data_processor