Acquisition

The Inscoper scanFRAP is integrated into the sequence for photomanipulation experiments. You will find a Photomanipulation dimension.

To use the Photomanipulation dimension:
  1. Select the scanning order.
    The ROI can be scanned one at a time (Sequential) or alternately (Alternate).
  2. Click on Add Manip. to add a photomanipulation event(protocol).
  3. Select the pre-configured channel (name) to be used during the sequences.
  4. Select all ROI to be bleached.
  5. Adjust the pulse time (corresponds to the time needed to the movement from the previous point and the exposure time).
  6. Adjust the number of iterations required.
  7. Adjust the laser power.
  8. Click on Confirm to validate all these settings.
    scanfrap_acquisition
    Note: Before starting a photomanipulation experiment, be sure that the scanFRAP is well calibrated.