Acquisition

Once the calibration and configuration are done, you can do the acquisition.

You will need to set your channel using the Multi-channel dimension. Thus you can set the FLIM and fluorescence channels.

Next you will find the FLIM dimension. In this dimension, depending on how many FLIM channels you set up, you will have 2 modes:

  • Simple mode: if you have set one FLIM channel during acquisition. Here you will set:
    • Gate Delay (the time interval between 2 temporal gates)
    • Delta T stack (B - the number of images to be measured by FLIM).
      fastflim_acquisition
  • Advanced mode: if you have more than one FLIM channel during acquisition. In this mode you can set:
    A. a specific Gate Delay and Delta T Size for each channel.
    B. Select the channel on which you wish to perform the FLIM measurement by checking the Do Flim box.
fastflim_acquisition_advanced

Once your FLIM experiment is set up, you have to click on Confirm to validate the setting. To finalize your acquisition sequence:

  1. Choose fastFLIM on the Data Processing and Charts drop-down list.
  2. By clicking on Edit Parameters you can set the minimum and maximum values for the Intensity Threshold for the FLIM calculation and click on Confirm.
  3. Indicate the path to save your images.
  4. Click on Start Acquisition.
    fastflim_start_acquisition