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Imaging
From core solution to custom techniques
Inscoper I.S.
Incorporating a specially-designed device, Inscoper I.S. provides a new user experience with improved technical performance, system integration and ease-of-use.
Acquisition
This tab allows you to set the entire acquisition sequence, using all the system's dimension modules.
Multichannels
This dimension is useful to select two (or more) channels and add them in the acquisition sequences.

Imaging
From core solution to custom techniques
- Inscoper I.S.
  Incorporating a specially-designed device, Inscoper I.S. provides a new user experience with improved technical performance, system integration and ease-of-use.
  - User journey
  - Getting started
    When you launch the Inscoper I.S. software, the start window will appear. You will have access to the software version number and can choose from several options to begin your user journey:
  - Display settings
    To access the interface settings, click on Settings > Display Settings.
  - Configuration
  - Acquisition
    This tab allows you to set the entire acquisition sequence, using all the system's dimension modules.
    - Time
      The Time dimension is used to run time-lapse and image cells over a long period of time.
    - Multichannels
      This dimension is useful to select two (or more) channels and add them in the acquisition sequences.
    - Positions
      This dimension is dedicated to image different XYZ positions from a sample. You can switch from this dimension to the Tiling dimension by the button Switch to Tiling.
    - Tiling
    - Preview feature in Position and Tiling dimension
    - Multiwell plate mode
      The Inscoper software has a strong feature for imaging multiwell plates and for some applications including High Content Imaging.The Multiwell plate mode of the Inscoper I.S. is compatible with all commercial or homemade supports (multi-well, multi-slide, etc.). An option Well Plate Editor allows you to simply add a large panel of supports. Click here to set it up.
    - Z-stack
      The volumetric imaging with Inscoper I.S. can be performed using the Z-Stack dimension.
    - Software autofocus
      The Inscoper I.S. has a software autofocus mode to keep samples at the focus throughout the acquisition sequence.
    - Multi-sequence acquisition
      When an acquisition sequence is ready, it is possible to launch it or to add others to create a multi-sequence acquisition.
    - Data Processor
    - Saving images and metadata
  - Visualization
- Custom Scripting module
  You can use this module to call a Python script during the acquisition sequence. You must have at your disposal python scripts, structured with a template similar to that of the applications included in the inscoper-scripts resource provided with this module. In this guide, you may select any script provided as part of this resource.
- scanFRAP
- TIRF
- liveDRIM
- liveSR
- liveRATIO
- MAICO I.S.
- fastFLIM

Multichannels

This dimension is useful to select two (or more) channels and add them in the acquisition sequences.

multichannels

Click on Add Channel.
Select it in the list of the pre-configured wanted channel and customize their settings according to the sample.
For each channel, you can apply some additional parameters:
- Z-stack: Apply z-stack dimension.
- Z-Offset: Apply a different offset if the focus varies.
- Shutter Blink: Optimize shutter control to limit sample exposure and prevent photobleaching.
- Camera: Select the one that you would like to use.
- Exposure(ms): Adjust exposure time.
- Time: Select the time interval at which you want to acquire the channel. You can choose to acquire it at all time points, only the first time point, only the last time point, or at a specific interval.
- Intensity: Adjust the excitation light (depending of the light source of your system).
- Delete your channel by clicking the red cross
Click on Live channel to have a preview of your settings.
Once you have finished editing the dimension, click on Confirm.

Note:
After adjusting the exposure time, click again on Live channel to update the image.

Note:
You can drag and drop channels to change their order.