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Create/Load a channel

create_load_channel

The expert users (if this option is activated) have access to all optical motorized elements of the microscope, like wheel filter, dichroic cube and light source. To manually create a fluorescent channel, you should follow the steps below:

  1. Select the right excitation source.
  2. Set its intensity.
  3. Adjust filter cube.
  4. Select the LightPath.
  5. If necessary, select brightfield light instead of or in addition to fluorescence.
  6. Name your channel.
  7. Save it:
    1. temporarily by clicking on Add (User Mode)
    2. permanently by clicking on Save (Expert Mode).
  8. The created channel will be available in the list.
Important: Some systems have more than one camera port. It is necessary to select the right one before imaging.