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Shading correction

Shading correction is a tool used to ensure uniform illumination of the field of view across the image. Perform this calibration using a chroma slide.


Performing a Shading Correction

  1. Click on Shading Correction.
  2. Specify the number of acquisitions — the shading correction image will be generated as the average of all captured images.


  3. Click Confirm to start the calibration
  4. Once the calibration is complete, you can:
    • Verify the result by clicking Test Shading in Live mode, or
    • View the generated shading image by clicking Show Shading Image.


  5. Click Confirm to validate the calibration.
  6. When the shading calibration is successfully done, the Shading Correction button changes from red to green.

Using shading correction by channel with the data processor

  1. Create your data processor (in Expert Mode) including the following nodes: Raw Data, Shading Correction, and Tiling.
  2. Apply the data processor after configuring your sequence (during the data processor and charts selection step).
  3. Click Edit Parameters.


  4. Select the channel(s) on which you want to apply the shading correction — it can be a single channel or all channels.
  5. Click Confirm to validate your selection.