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Imaging
From core solution to custom techniques
Inscoper I.S.
Incorporating a specially-designed device, Inscoper I.S. provides a new user experience with improved technical performance, system integration and ease-of-use.
Acquisition
This tab allows you to set the entire acquisition sequence, using all the system's dimension modules.
Dimensions
Software autofocus
The Inscoper I.S. has a software autofocus mode to keep samples at the focus throughout the acquisition sequence.

Imaging
From core solution to custom techniques
- Inscoper I.S.
  Incorporating a specially-designed device, Inscoper I.S. provides a new user experience with improved technical performance, system integration and ease-of-use.
  - User journey
  - Getting started
    When you launch the Inscoper I.S. software, the start window will appear. You will have access to the software version number and can choose from several options to begin your user journey:
  - Display settings
    To access the interface settings, click on Settings > Display Settings.
  - Configuration
  - Acquisition
    This tab allows you to set the entire acquisition sequence, using all the system's dimension modules.
    - Dimensions
      - Time
        The Time dimension is used to run time-lapse and image cells over a long period of time.
      - Multichannels
        This dimension is useful to select two (or more) channels and add them in the acquisition sequences.
      - Positions
        This dimension is dedicated to image different XYZ positions from a sample. You can switch from this dimension to the Tiling dimension by the button Switch to Tiling.
      - Tiling
      - Multiwell plate mode
        The Inscoper software has a strong feature for imaging multiwell plates and for some applications including High Content Imaging.The Multiwell plate mode of the Inscoper I.S. is compatible with all commercial or homemade supports (multi-well, multi-slide, etc.). An option Well Plate Editor allows you to simply add a large panel of supports. Click here to set it up.
      - Z-stack
        The volumetric imaging with Inscoper I.S. can be performed using the Z-Stack dimension.
      - Software autofocus
        The Inscoper I.S. has a software autofocus mode to keep samples at the focus throughout the acquisition sequence.
      - Multi-sequence acquisition
        When an acquisition sequence is ready, it is possible to launch it or to add others to create a multi-sequence acquisition.
      - Data Processor
      - Saving images and metadata
    - Data export
    - Semi-automated feedback microscopy feature
      The Inscoper I.S. can be used to perform some feedback microscopy experiments. For example, you can image a large sample using the tiling dimension. Then you can select a few areas of interest on this large image and automatically import them into a new sequence.
  - Visualization
- scanFRAP
- TIRF
- fastFLIM
- liveRATIO
- MAICO I.S.

Edit online

Software autofocus

The Inscoper I.S. has a software autofocus mode to keep samples at the focus throughout the acquisition sequence.

Select the number of focus to perform to each position.
Select the step (µm) between each focus.
Select the channel to use.
Select the camera to use and its exposure time.
Select the autofocus algorithms to be applied: maximal intesity or sharpness (the best focus plane will be the sharpest).
Add Condition: interval of autofocus depending on dimension (for example, every 3 time points).
Check Advanced Parameters to set up the device to do the autofocus (for example, the piezo or the focus of the microscope) and if you need to apply offset to another device.
Test the autofocus protocol.
Click on Confirm.

Important: The Autofocus dimension must be executed after the active dimension during an acquisition sequence.