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Inscoper I.S.
Incorporating a specially-designed device, Inscoper I.S. provides a new user experience with improved technical performance, system integration and ease-of-use.
Acquisition
This tab allows you to set the entire acquisition sequence, using all the system's dimension modules.
Dimensions
Autofocus
The Inscoper I.S. software has an auto-focus mode to keep samples at the perfect focus throughout the acquisition sequence.

Solutions
From core solution to custom techniques
- Inscoper I.S.
  Incorporating a specially-designed device, Inscoper I.S. provides a new user experience with improved technical performance, system integration and ease-of-use.
  - User journey
  - Getting started
    When you launch the Inscoper I.S. software, the start window will appear. You will have access to the software version number and can choose from several options to begin your user journey:
  - Display settings
    To access the interface settings, click on Settings > Display Settings.
  - Configuration
  - Acquisition
    This tab allows you to set the entire acquisition sequence, using all the system's dimension modules.
    - Dimensions
      - Time
        The Time dimension is used to run time-lapse and image cells over a long period of time.
      - Multichannels
        This dimension is useful to select two (or more) channels and add them in the acquisition sequences.
      - Positions
        This dimension is dedicated to image different XYZ positions from a sample. You can switch from this dimension to the Tiling dimension by the button Switch to Tiling.
      - Tiling
      - Multiwell plate mode
        The Inscoper software has a strong feature for imaging multiwell plates and for some applications including High Content Imaging.
      - Automated Focus Map
        The Automated Focus Map is a software optional feature of the Inscoper I.S. that automatically generates a relief map of the biological sample when performing tiling imaging.
      - Autofocus
        The Inscoper I.S. software has an auto-focus mode to keep samples at the perfect focus throughout the acquisition sequence.
    - Multi-sequence acquisition
      When an acquisition sequence is ready, it is possible to launch it or to add others to create a multi-sequence acquisition.
    - Data Processor
    - Saving images and metadata
  - Visualization
  - Data export
  - Semi-automated feedback microscopy feature
    The Inscoper I.S. can be used to perform some feedback microscopy experiments. For example, you can image a large sample using the tiling dimension. Then you can select a few areas of interest on this large image and automatically import them into a new sequence.
- scanFRAP
- fastFLIM
- liveRATIO
- MAICO I.S.

Autofocus

The Inscoper I.S. software has an auto-focus mode to keep samples at the perfect focus throughout the acquisition sequence.

Select the number of focus to perform to each position.
Select the step (µm) between each focus.
Specify the frequency of autofocus in the acquisition sequence:
1. Interval: number of images taken between each autofocus.
2. Autofocus group:
  - GLOBAL - applying autofocus on all images
  - POSITION - applying autofocus on a set of groupe size (XY) positions
  - TILING - applying autofocus on all tiling groups
3. Group size: number of positions to which the found focus value is applied.
Select the channel to use.
Select the camera to use and its exposure time.
Select the autofocus algorithms to be applied : maximal intesity or sharpness (the best focus plane will be the sharpest).
Test the autofocus protocol.
Click on Confirm.
Important: The Autofocus dimension must be executed after the active dimension during an acquisition sequence.