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Inscoper I.S.
Incorporating a specially-designed device, Inscoper I.S. provides a new user experience with improved technical performance, system integration and ease-of-use.
Acquisition
This tab allows you to set the entire acquisition sequence, using all the system's dimension modules.
Dimensions
Automated Focus Map
The Automated Focus Map is a software optional feature of the Inscoper I.S. that automatically generates a relief map of the biological sample when performing tiling imaging.
Automated Focus Map - Simple tiling

Solutions
From core solution to custom techniques
- Inscoper I.S.
  Incorporating a specially-designed device, Inscoper I.S. provides a new user experience with improved technical performance, system integration and ease-of-use.
  - User journey
  - Getting started
    When you launch the Inscoper I.S. software, the start window will appear. You will have access to the software version number and can choose from several options to begin your user journey:
  - Display settings
    To access the interface settings, click on Settings > Display Settings.
  - Configuration
  - Acquisition
    This tab allows you to set the entire acquisition sequence, using all the system's dimension modules.
    - Dimensions
      - Time
        The Time dimension is used to run time-lapse and image cells over a long period of time.
      - Multichannels
        This dimension is useful to select two (or more) channels and add them in the acquisition sequences.
      - Positions
        This dimension is dedicated to image different XYZ positions from a sample. You can switch from this dimension to the Tiling dimension by the button Switch to Tiling.
      - Tiling
      - Multiwell plate mode
        The Inscoper software has a strong feature for imaging multiwell plates and for some applications including High Content Imaging.
      - Automated Focus Map
        The Automated Focus Map is a software optional feature of the Inscoper I.S. that automatically generates a relief map of the biological sample when performing tiling imaging.
        Automated Focus Map - Simple tiling
        Automated Focus Map - Multiple area tiling
        Automated Focus Map - Multiwell plate
      - Autofocus
        The Inscoper I.S. software has an auto-focus mode to keep samples at the perfect focus throughout the acquisition sequence.
    - Multi-sequence acquisition
      When an acquisition sequence is ready, it is possible to launch it or to add others to create a multi-sequence acquisition.
    - Data Processor
    - Saving images and metadata
  - Visualization
  - Data export
  - Semi-automated feedback microscopy feature
    The Inscoper I.S. can be used to perform some feedback microscopy experiments. For example, you can image a large sample using the tiling dimension. Then you can select a few areas of interest on this large image and automatically import them into a new sequence.
- scanFRAP
- fastFLIM
- liveRATIO
- MAICO I.S.

Automated Focus Map - Simple tiling

In the tiling dimension, click on Add position to create the tiling.
Click on Edit to customize the tiling and adjust the Focus Map.
Click on Auto to configure the Focus Map.
Choose the step and size of the stack.
You can select an existing pattern from the drop-down list (after that go to the step 9) OR you can create a new pattern by clicking on Edit.
To create a new pattern, click Edit choose the pattern you want: Grid or Random:
1. For Grid, indicate the number of image you want to acquire horizontally and vertically, and the Gap (distance between 2 images).
  The offset X and Y allows you to move your pattern in the well.
2. If you choose a Random, indicate the position number and the minimal distance between positions.
Save your pattern by clicking on Add.
Come back to the dimension by clicking on Switch to Tiling.
Choose the channel to acquire the stack.
You can apply some filters by checking the box Filter unsure position:
- Filter Radius: filter to smooth the noise by replacing each pixel by the median of the neighbor (0= no smoothing, 1= median on 3x3 square around the pixel, 2= median on 5x5 square around the pixel, …).
- BackgroundRoi: automatic calculation of Background and BackgroundDelta values by drawing an ROI. Background will be the mean value of the ROI and BackgroundDelta will be the double of the standard deviation.
- Background: background mean value.
- Background Delta: amplitude of the noise.
- Valid pixels: percent of pixel that should be far of the background to validate the position
Click on Start.

System will perform all points to create the Focus Map and you will obtain a list of points. By clicking on Goto or on the image, you can check the focus.
Click on the Confirm, the Focus Map is ready to use. You can continue to configure your acquisition sequence.