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Imaging
From core solution to custom techniques
Inscoper I.S.
Incorporating a specially-designed device, Inscoper I.S. provides a new user experience with improved technical performance, system integration and ease-of-use.
Acquisition
This tab allows you to set the entire acquisition sequence, using all the system's dimension modules.
Dimensions
Tiling
Focus Map automated setup for simple and multiple tiling
The Automated Focus Map is a software optional feature of the Inscoper I.S. that automatically generates a relief map of the biological sample when performing tiling imaging. There are 2 types of the Automated Focus Map :

Imaging
From core solution to custom techniques
- Inscoper I.S.
  Incorporating a specially-designed device, Inscoper I.S. provides a new user experience with improved technical performance, system integration and ease-of-use.
  - User journey
  - Getting started
    When you launch the Inscoper I.S. software, the start window will appear. You will have access to the software version number and can choose from several options to begin your user journey:
  - Display settings
    To access the interface settings, click on Settings > Display Settings.
  - Configuration
  - Acquisition
    This tab allows you to set the entire acquisition sequence, using all the system's dimension modules.
    - Dimensions
      - Time
        The Time dimension is used to run time-lapse and image cells over a long period of time.
      - Multichannels
        This dimension is useful to select two (or more) channels and add them in the acquisition sequences.
      - Positions
        This dimension is dedicated to image different XYZ positions from a sample. You can switch from this dimension to the Tiling dimension by the button Switch to Tiling.
      - Tiling
        Tiling creation
        The tiling tool is used to image large samples. You can switch from this dimension to the Positions dimension by the top button Switch to Positions.
        Tiling edition
        Focus Map manual setup
        The Focus Map is a feature dedicated to adapt the focus during the acquisition when imaging samples that are not flat.
        Focus Map automated setup for simple and multiple tiling
        The Automated Focus Map is a software optional feature of the Inscoper I.S. that automatically generates a relief map of the biological sample when performing tiling imaging. There are 2 types of the Automated Focus Map :
      - Multiwell plate mode
        The Inscoper software has a strong feature for imaging multiwell plates and for some applications including High Content Imaging.The Multiwell plate mode of the Inscoper I.S. is compatible with all commercial or homemade supports (multi-well, multi-slide, etc.). An option Well Plate Editor allows you to simply add a large panel of supports. Click here to set it up.
      - Z-stack
        The volumetric imaging with Inscoper I.S. can be performed using the Z-Stack dimension.
      - Software autofocus
        The Inscoper I.S. has a software autofocus mode to keep samples at the focus throughout the acquisition sequence.
      - Multi-sequence acquisition
        When an acquisition sequence is ready, it is possible to launch it or to add others to create a multi-sequence acquisition.
      - Data Processor
      - Saving images and metadata
    - Data export
    - Semi-automated feedback microscopy feature
      The Inscoper I.S. can be used to perform some feedback microscopy experiments. For example, you can image a large sample using the tiling dimension. Then you can select a few areas of interest on this large image and automatically import them into a new sequence.
  - Visualization
- scanFRAP
- TIRF
- fastFLIM
- liveRATIO
- MAICO I.S.

Focus Map automated setup for simple and multiple tiling

The Automated Focus Map is a software optional feature of the Inscoper I.S. that automatically generates a relief map of the biological sample when performing tiling imaging. There are 2 types of the Automated Focus Map :

Simple tiling;
Multiple tiling.

To start configuring:
1. simple tiling: click on Auto in Tiling Dimension.
2. multiple tiling: click on Focus Map Start button in Tiling Dimension.
The following parameters must be set for both simple and multiple tiling:
1. Choose the step and size of the stack.
2. Select an existing pattern or click Edit to create a new one.
3. If Edit is selected:
  - Grid: set number of images (horizontal and vertical), gap, offset X and Y. The offset X and Y allows you to move your pattern in the well.
  - Random: set number of positions and minimum distance between them.
4. Click Add to save the pattern.
5. Return to the Dimension via Switch to Tiling.
6. Select channel to acquire the stack.
7. Optional: Check Filter unsure position and set parameters:
  - Filter Radius: filter to smooth the noise by replacing each pixel by the median of the neighbor (0= no smoothing, 1= median on 3x3 square around the pixel, 2= median on 5x5 square around the pixel, …).
  - BackgroundRoi: ROI for auto-calculating background values.
  - Background: Mean background value.
  - Background Delta: Noise amplitude.
  - Valid Pixels: Percentage needed to validate position.
If using multiple tiling: select the tiling area for which you want to create a Focus Map
Click on Start. The system will automatically scan all points to generate the Focus Map. A list of focus points will be displayed. When the Focus Map is ready: A blue circle will appear on the right side of each position and in the bottom-right corner, near the Confirm button. You will be automatically redirected to the Tiling Dimension screen. When the blue circle reaches 100%, the Focus Map is complete.
Click Confirm to validate and proceed. You can continue to configure your acquisition sequence.