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Imaging
From core solution to custom techniques
Inscoper I.S.
Incorporating a specially-designed device, Inscoper I.S. provides a new user experience with improved technical performance, system integration and ease-of-use.
Acquisition
This tab allows you to set the entire acquisition sequence, using all the system's dimension modules.
Dimensions
Tiling
Tiling edition

Imaging
From core solution to custom techniques
- Inscoper I.S.
  Incorporating a specially-designed device, Inscoper I.S. provides a new user experience with improved technical performance, system integration and ease-of-use.
  - User journey
  - Getting started
    When you launch the Inscoper I.S. software, the start window will appear. You will have access to the software version number and can choose from several options to begin your user journey:
  - Display settings
    To access the interface settings, click on Settings > Display Settings.
  - Configuration
  - Acquisition
    This tab allows you to set the entire acquisition sequence, using all the system's dimension modules.
    - Dimensions
      - Time
        The Time dimension is used to run time-lapse and image cells over a long period of time.
      - Multichannels
        This dimension is useful to select two (or more) channels and add them in the acquisition sequences.
      - Positions
        This dimension is dedicated to image different XYZ positions from a sample. You can switch from this dimension to the Tiling dimension by the button Switch to Tiling.
      - Tiling
        Tiling creation
        The tiling tool is used to image large samples. You can switch from this dimension to the Positions dimension by the top button Switch to Positions.
        Tiling edition
        Focus Map manual setup
        The Focus Map is a feature dedicated to adapt the focus during the acquisition when imaging samples that are not flat.
        Focus Map automated setup for simple and multiple tiling
        The Automated Focus Map is a software optional feature of the Inscoper I.S. that automatically generates a relief map of the biological sample when performing tiling imaging. There are 2 types of the Automated Focus Map :
      - Multiwell plate mode
        The Inscoper software has a strong feature for imaging multiwell plates and for some applications including High Content Imaging.The Multiwell plate mode of the Inscoper I.S. is compatible with all commercial or homemade supports (multi-well, multi-slide, etc.). An option Well Plate Editor allows you to simply add a large panel of supports. Click here to set it up.
      - Z-stack
        The volumetric imaging with Inscoper I.S. can be performed using the Z-Stack dimension.
      - Software autofocus
        The Inscoper I.S. has a software autofocus mode to keep samples at the focus throughout the acquisition sequence.
      - Multi-sequence acquisition
        When an acquisition sequence is ready, it is possible to launch it or to add others to create a multi-sequence acquisition.
      - Data Processor
      - Saving images and metadata
    - Data export
    - Semi-automated feedback microscopy feature
      The Inscoper I.S. can be used to perform some feedback microscopy experiments. For example, you can image a large sample using the tiling dimension. Then you can select a few areas of interest on this large image and automatically import them into a new sequence.
  - Visualization
- scanFRAP
- TIRF
- fastFLIM
- liveRATIO
- MAICO I.S.

Tiling edition

tiling_type

Choose the way to generate your tiling and set its specific parameters:
1. For the Diagonale setup:
  - Move the stage to the top left corner of the structure of interest and click on Get for the first position.
  - Move the stage to the bottom right corner of the structure of interest and click on Get for the second position.
2. For the Center setup:
  - Set the position of the center of your tiling.
  - Set the width and the height of your tiling.
Select an appropriate overlap pourcentage (10% is usually enough for final stitching for example) OR check Automode which allows to calculate the exact overlap between tiles to respect the 2 coordinates chosen for the tiling creation.
If needed, adjust the Custom Step (distance between the tiles).
Select the acquisition order of tiles.
Fastpath: Snake mode is recommended for the screening mode because of its speed. This mode is optimized when the acquisition needs to be done fastly.
Edit the Focus map if you need to use it.
Click on Confirm.

Note: Users can remove the Focus checkbox if they don’t want to consider the Z coordinate used for the tiling creation.

Note: The GoTo button can be used to move the stage to the previously saved position.